| Product Name | Size | Price | Stock |
|---|---|---|---|
| Anti-HMGB1 antibody SKU:ab18256 | 100µg |
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|
Lanes 1 - 4: Merged signal (red and green). Green - ab18256 observed at 29 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Controlab9484, observed at 37 kDa.
ab18256 was shown to recognize HMGB1 in wild-type HAP1 cells as signal was lost at the expected MW in HMGB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HMGB1 knockout samples were subjected to SDS-PAGE. ab18256 and Anti-GAPDH antibody [mAbcam 9484] - Loading Controlab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbedab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbedab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:Western blot - Anti-HMGB1 antibody (ab18256) at 1 µg/mL
Lane 1:Wild-type HAP1 whole cell lysate at 20 µg
Lane 2:HMGB1 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 24 kDa
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