TaKaRa MiniBEST Universal RNA Extraction Kit is designed for the rapid, small-scale preparation of highly pure total RNA from cultured cells, animal tissues and plant tissues. The kit use an unique cell lysis buffer to achieve isolation of RNA rapidly and conveniently. The procedure avoids the phenol and chloroform extraction. Cells or tissues are lysed by incubation in the lysis buffer after homogenization or grinding in the presence of liquid nitrogen. With the gDNA Eraser Spin Column (for remove the genome DNA) and RNA Spin Column (for binding RNA), highly pure and quality RNA can be obtained. The protocol provides a simple method to achieve the rapid isolation of highly pure RNA and the entire procedure can be accomplished within 20 minutes after tissues or cells being lysed. The pure RNA obtained by the kit basically contain no protein and have no genomic DNA contamination. The protocol allows the purification of about 10 - 30 mg of high quality of RNA from 1.0 x 105 - 1.0 x 107 cultured cells , 5 - 40mg of mammalian tissues, and 50 - 100 mg plant tissues. The highly pure RNA eluted with RNase-free H2O can be used in Northern blot, dot blot, mRNA purification, in vitro translation, RNase protection assay, RT-PCR, real time RT-PCR, cDNA library construction and other kinds of molecular experiments. Using the gDNA Eraser Spin Column can isolate easily genome DNA which can be used in PCR reaction and other kinds of molecular experiments