Product name

EdU DNA Synthesis Monitoring Kit Flow Cytometry

Detection method

Flow cytometry-fluorescent

Sample type

Adherent cells, Suspension cells

Assay type

Cell-based

Assay duration

Multiple steps standard assay

Species reactivity

Reacts with: Mammals

Product overview

The EdU DNA Synthesis Monitoring Kit ab287859 (previously known as EZClick EdU DNA Synthesis Monitoring Kit, K946) utilizes a novel approach that relies on incorporation of 5-EdU (5-ethynyl-2’deoxyuridine) as nucleoside analog to thymidine into newly synthesized DNA directly in the cell culture. Incorporation of EdU into genomic DNA in S-phase is detected based on a click reaction between the alkyne moiety of EdU and fluorescent azide. Compared to historically used BrdU, click reaction is carried in mild conditions and flow cytometry/fluorescence microscopy can be used for assessment of proliferating cells in the population. Our kit provides sufficient materials for 100 assays based on the protocol below.

Applications:

• Detection of DNA synthesis in proliferating cells and assessment of cell cycle phase


• Screening for genotoxic compounds and effectors of cell division cycle


• Evaluating effects of anti-cancer drugs and genotoxic agents


 

Tested applications

Suitable for: Flow Cytmore details

Platform

Flow cytometer, Fluorescence microscope