Product name

Recombinant Human Alpha-synuclein protein aggregate (Active)
See all Alpha-synuclein proteins and peptides

Biological activity

Endogenous alpha-synuclein phosphorylation.

100 µM alpha synuclein protein monomer (ab218818) seeded with 10 µM alpha synuclein protein aggregate (ab218819) in 25 µM Thioflavin T (ab120751) (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours.

Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.

Endotoxin Level: 10-20 EU/mL

Purity

> 95 % SDS-PAGE.
ab218819 was purified by ion-exchange.

Endotoxin level

<=20.000 Eu/ml

Expression system

Escherichia coli

Accession

P37840

Protein length

Full length protein

Animal free

No

Nature

Recombinant

• Species

  Human

• Sequence

  MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

• Predicted molecular weight

  14 kDa

• Amino acids

  1 to 140

• Additional sequence information

  (NP_000336.1) (GeneID 6622)

Description

Recombinant human Alpha-synuclein protein (Active)