Product name

Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
 

Product overview

Mouse and Rabbit specific HRP/DAB (ABC) Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.

It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.

In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB.

Notes

For alternative streptavidin-biotin IHC detection kits see:
- Anti-Rabbit HRP-DAB IHC kit ab64261
- Anti-Mouse HRP-DAB IHC Kit ab64259

For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469

Alternatively, see HRP-AEC IHC detection kits.

For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Tested applications

Suitable for: IHC-P