Product name

ATP Assay Kit (Colorimetric/Fluorometric)
 

Detection method

Colorimetric/Fluorometric

Sample type

Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate

Assay type

Quantitative

Sensitivity

< 1 µM

Assay time

1h 00m

Product overview

ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) use a robust, simple method; the ATP assay protocol relies on the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (ODmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

This kit can detect as low as 1 µM of ATP in various samples.

ATP assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader

Notes

This product is manufactured by BioVision, an Abcam company and was previously called K354 ATP Colorimetric/Fluorometric Assay Kit. K354-100 is the same size as the 100 test size of ab83355.