Product name

Mouse on Mouse Polymer IHC Kit

Sample type

Tissue

Species reactivity

Reacts with: Mouse

Product overview

Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.

The mouse on mouse kit includes an Fab'anti-mouse IgG(H+L) blocking reagent and an HRP polymer conjugated Fab'anti-mouse IgG(H+L) detection reagent.

This kit replaces mouse on mouse kit ab127055. The reagents used in this kit have been carefully optimized to match the performance of ab127055 (see validation images below). This kit does not include hydrogen peroxide blocking reagent: we recommend hydrogen peroxide blocking reagent ab64218.

In the mouse on mouse IHC protocol:

- Cover the tissue section with the blocking reagent (before the addition of the mouse primary antibody) for 30-60 minutes. Wash 2-3 times.
The blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites.

- Incubate with primary antibody of your choice raised in mouse. Wash 2-3 times.

- Incubate with HRP polymer detector reagent for ~15 minutes. Wash 2-3 times.

- Stain with a chromogen (usually DAB, eg. ab64238) for detection. 

Otherwise, follow a conventional IHC protocol for DAB staining.

The detector and blocker have <1% reactivity to human and rabbit antibodies.

Notes

Other products for IHC

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Tested applications

Suitable for: IHC-P