| Product Name | Size | Price | Stock |
|---|---|---|---|
| Human D-Dimer ELISA Kit SKU:ab260076 | 1 x 96 tests |
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|
Human D-Dimer ELISA Kit (ab260076) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of D-Dimer protein in hep plasma, serum, urine, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human D-Dimer with 2.36 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
Plasma collected with EDTA is not compatible with this kit.
ASSAY SPECIFICITY
This kit recognizes native human D-Dimer protein in serum, plasma (citrate), plasma (heparin), cell and tissue culture supernatant, and urine samples only.
Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY
Recombinant human fibrinogen, plasminogen, angiostatin, tPA, PAI1, and plasmin were prepared at 150 ng/mL, diluted serially, and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE
Recombinant human fibrinogen, plasminogen, angiostatin, tPA, PAI1, and plasmin were prepared at 150 ng/mL, diluted serially, and assayed for interference with human D-Dimer. No cross-reactivity was observed.
SPECIES REACTIVITY
This kit recognizes human D-Dimer protein.
Other species reactivity was determined by measuring 1: 200 mouse, rat, and bovine serum samples, interpolating the D-Dimer protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the D-Dimer protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
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