Human Insulin ELISA kit (ab278123) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Insulin protein in human serum, plasma heparin, and plasma EDTA samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Insulin with 7.13 pmol/L sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

        -Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips