TaKaRa Taq DNA Polymerase and premix

TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.

For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+). A convenient 2X PCR master mix of Takara Taq enzyme, buffer, and dNTP mixture is available as Premix Taq DNA Polymerase (TaKaRa Taq Version 2.0) (Cat. # R004A).

Two other formulations of TaKaRa Taq DNA polymerase are also available:

• Several antibody-mediated hot-start versions of Takara Taq—for preventing non-specific amplification from mispriming during reaction setup
• GMP-grade version—for an added level of confidence in our quality polymerase

Overview

• Excellent for standard PCR amplifications
• Low bacterial DNA contamination
• Premix Taq enables easy PCR setup and minimizes pipetting steps

Applications

• Routine PCR
• DNA sequencing

Source

• Recombinant E. coli

PCR products

The resulting PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine (A) to the 3' end. It is also possible to clone the PCR product into blunt-end vectors after blunting and phosphorylation of the ends.