PrimeSTAR Max DNA Polymerase

A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.

Overview

• Highest fidelity of any commercially available DNA polymerase
• Fastest extension speed means less time required for PCR cycles
• Convenient 2X premix includes buffer and dNTPs, allowing reaction assembly in less time
• Antibody-mediated hot-start formulation
• Proven performance as reported in peer-reviewed literature

Applications

• Fast PCR for high-throughput studies
• Cloning and expression studies
• Structure/function studies
• Evolutionary studies (e.g., SNP analyses)

PCR product termini

• PrimeSTAR Max DNA Polymerase produces products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Recommended maximum amplicon length

• Up to 6 kb (human genomic DNA template or human cDNA template)
• Up to 10 kb (E. coli genomic DNA template)
• Up to 15 kb (lambda DNA template)