Product name

LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)
 

Detection method

Colorimetric

Sample type

Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts

Assay type

Enzyme activity (quantitative)

Sensitivity

> 1 mU/ml

Range

1 mU/ml - 100 mU/ml

Assay time

0h 30m

Product overview

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.

LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37ºC