• Product name

  Human CD276 knockout HEK-293T cell line
   

• Parental Cell Line

  HEK293T

• Organism

  Human

• Mutation description

  Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2

• Passage number

  <20

• Knockout validation

  Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB)

• Tested applications

  Suitable for: WB, ICC, Flow Cyt

• Biosafety level

          2

• General notes

  Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

  Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

  Culture medium: DMEM (High Glucose) + 10% FBS

  Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.