Product name

COVID-19 (SARS-COV-2) human knockout cell lines panel

Product overview

A collection of 5 knockout cell lines to targets in the cell entry and cytokine storm pathways, in one kit for your convenience, with matching parental cell lines. For Research Use Only

Included in this panel:

ACE-2 knockout CACO-2 cell line (ab273731) (Unfortunately we are currently unable to provide the CACO-2 WT cell line. We would recommend using the ATCC CACO-2 WT)

TMPRSS2 knockout Calu-3 cell line (ab273734) + recommended control (ab275465)

CSF3 knockout K562 cell line (ab273740) + recommended control (ab275469)

IL-6 knockout A549 cell line (ab273751) + recommended control (ab275463)

TNF knockout THP-1 cell line (ab273761) + recommended control (ab275477)

Target

ACE-2: SARS-COV-2 binds to ACE-2 receptor via spike protein to allow cell entry (Pubmed: 32015507)

TMPRSS2: TMPRSS2 cleaves spike at the S2’ site and is shown to be essential for activation of SARS-COV-2 in Calu-3 cells through antisense-mediated knockdown of TMPRSS2 expression (doi: https://doi.org/10.1101/2020.04.15.042085)

CSF3: upregulated in patients with severe COVID-19 symptoms. Found to be the most upregulated gene by SARS-COV-2 (Pubmed: 33551833)

IL-6 and TNF: The cytokine storm in COVID-19 shows increased levels of IL-6 and TNF. (Pubmed: 32754163)

Notes

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.