PrimeSTAR GXL DNA Polymerase

 PrimeSTAR GXL DNA Polymerase provides efficient PCR amplification even for the toughest scenarios, including amplification of GC-rich targets, amplification in   the presence of excess template that can contribute to nonspecific binding, and amplification of long products (up to 30 kb). The antibody-mediated hot-start feature   enhances PCR specificity and sensitivity.

 PrimeSTAR GXL polymerase comes with separate tubes of 5X buffer and dNTP mixture.PrimeSTAR GXL polymerase is our most robust high-fidelity polymerase   available for challenging targets (GC-rich sequences, excess template, long amplicons).

Overview

• Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
• Antibody-mediated hot-start formulation
• High tolerance for GC-rich templates as well as the presence of excess nucleic acids templates

Applications

• Reliable amplification of long, GC-rich, or AT-rich sequences, such as regulatory regions and repeats
• HLA typing
• Unbiased library amplification for NGS

Termini of PCR products

• PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Recommended maximum amplicon length

• Up to 30 kb (human genomic DNA template)
• Up to 40 kb (lambda DNA template)
• Up to 13.5 kb (human cDNA template)