TB Green Premix Ex Taq (Tli RNase H Plus)

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

Overview

• Lower Cq values as a result of fast extension rates
• Use with broad range of amplicon sizes, even over 500 bp
• Convenient 2X master mix is excellent for high-speed qPCR
• High sensitivity: detects fewer than 100 copies
• Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
• Compatible with all commonly used real-time PCR (qPCR) instruments
• Minimizes qPCR inhibition due to residual mRNA in input cDNA
• Includes TB Green for intercalator-based qPCR

Applications

• TB Green qPCR using TB Green and ROX Reference Dye
• Gene expression analysis
• Genotyping by real-time PCR
• High-throughput real-time PCR
• Amplification of long products