Premix Ex Taq master mix for probe-based real-time PCR

Premix Ex Taq (Probe qPCR) is a 2X master mix for real-time PCR (qPCR) using probe-based qPCR or 5' nuclease assays. This 2X master mix includes Takara Ex Taq HS—a hot-start PCR enzyme in combination with anti-Taq antibody—in a qPCR-optimized buffer. Takara Ex Taq HS inhibits non-specific amplification while enabling high-efficiency amplification and detection sensitivity during real-time PCR analyses. Additionally, Tli RNase H, a heat-resistant RNase enzyme, is included in the real-time PCR premix in order to minimize PCR inhibition due to the presence of residual mRNA in the input cDNA. This master mix is ideal for high-speed PCR, allows accurate target quantification and detection over a broad dynamic range, and enables highly reproducible and reliable real-time PCR analyses.

Overview

• Rapid and accurate PCR product detection
• Quantitative gene expression analysis using real-time PCR (qPCR)
• 2X master mix enables easy pipetting
• Optimized buffer increases amplification efficiency, highly sensitive probe-based qPCR assays, and 5' nuclease assays
• Included Tli RNase H minimizes PCR inhibition by removing residual mRNA in input cDNA

Applications

• Real-time PCR (qPCR) quantification of DNA using methods such as probe-based qPCR assays and 5' nuclease assays

Components

• Two tubes of ROX Reference Dye are supplied separately with Cat. # RR390A/B and RR390L/W. The ROX Dye serves as a convenient internal reference standard when normalizing signals resulting from non-PCR fluorescence fluctuations that occur between wells or over time in different instruments.
• Cat. # RR390L/W are larger package sizes—5 ml and 25 ml (5 x 5 ml), respectively—of Premix Ex Taq (Probe qPCR). Each of these items provides convenient larger packaging and is supplied with a single, separate vial of ROX Reference Dye.
• Cat. # RR39LR/WR are larger package sizes—5 ml and 25 ml (5 x 5 ml), respectively—that already contain ROX Reference Dye in the real-time PCR Premix.