Product name

Anti-GFP antibody
 

Description

Rabbit polyclonal to GFP

Host species

Rabbit

Specificity

Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

GFP antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

Tested applications

Suitable for: ELISA, IHC-Fr, ICC, IHC-P, IP, WB, IHC-FoFr, IHC-FrFl, Electron Microscopymore details

Species reactivity

Reacts with: Species independent

Immunogen

Recombinant full length protein corresponding to GFP. Green fluorescent protein (GFP) from Aequorea victoria.
Database link: P42212

Positive control

• The Recombinant A. victoria GFP protein (ab84191), any other purified recombinant GFP, any cell line confirmed to overexpress GFP. ICC: NIH3T3, U2OS and glandular stomach cells. IHC: Mouse brain and dog heart tissue. WB: Sample: COS7 and LNCaP whole cell lysate - transfected with GFP-Eml4.

General notes

The total IgG concentration has been determined to be 5 mg/mL. The specific IgG concentration is unknown. This product should be kept refrigerated at all times whilst in short term storage. Using sterilised equipment will reduce the risk of bacterial contamination. 

Anti-GFP antibody (ab6556) is the purified version of this antibody (see Related Products)